Not known Details About attune flow cytometer
Not known Details About attune flow cytometer
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Knowledge-enriched characterization of cell populations. Morphological details from images can add into the richness of flow cytometry info. As an example, the determine reveals an in any other case standard apoptosis assay making use of Annexin V and PI, adding mobile imaging to characterize cells in Each and every population to expose morphologically distinctive characteristics. These insights could not are actually received from multiplex staining on your own.
As samples are acquired around the Attune CytPix Flow Cytometer, the large-pace brightfield digicam captures and shops pictures of detected occasions in a charge around 6,000 pictures for each next, depending on the flow amount and image measurement. For increased adaptability, Attune Cytometric Software program helps you to regulate the impression seize frequency as needed and choose a selected gate for image capture, if essential.
Mix acoustic concentrating know-how with superior-parameter Examination for flexible spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating that has a high-pace brightfield digital camera for simultaneous high throughput flow cytometry and substantial resolution brightfield imaging.
"A picture just isn't rather really worth a thousand dots, however it truly helps you to see photos. It is excellent to view pictures that correlate with staining. By way of example, AnnexinV-favourable cells that happen to be morphologically unique than healthier cells, or CD14+ monocytes which have been much larger and even more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is actually illuminating to check out what number of doublets slip via traditional singlet gates, and terrific to verify the status of unforeseen double-optimistic occasions in my Assessment."
Optimizing gating tactic. Even strong guide singlet gating is error-prone and remains a subjective final decision issue in Pretty much all flow cytometry assays. Imaging can be utilized to verify and adjust gates to incorporate only solitary cells of desire.
Steady image high-quality even at high throughput Acoustic concentrating and also a substantial-pace digital camera Blend to graphic these T cells continuously at very low or significant flow prices. Quickly regulate target and digital camera settings to meet experimental necessities.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can trust in for reliable and reliable efficiency. The Attune CytPix design incorporates a significant-velocity brightfield digital camera and AI-pushed picture analysis software, with modern updates like machine Mastering applications and an intuitive user interface, enabling Improved information precision, accuracy, and faster insights into sample biology.
Most likely most of all, these situations contain cells of Obviously distinct phenotypes which could lead to incorrect conclusions concerning double positive activities (especially in unusual populations).
AI-driven automated graphic analysis Along with the Attune Cytometric Software package translates occasion features into distinct morphology parameters which might be coupled with typical fluorescence and scatter parameters to reveal hugely reproducible populace modifications and distinctive subsets.
We Earlier shown the power of imaging Automobile-T/Ramos mobile interactions. Permit’s check out just the population of greatest desire, the double favourable functions, to learn more. We will now use prolonged morphological parameters (below, circularity and skewness of depth) to further take a look at the options of these populations and refine gating on these activities, expanding data robustness.
Leverage the strength of Highly developed technological know-how and innovation to accelerate your discoveries. Trust in the Attune Flow Cytometer family members to deliver the trustworthiness, pace, and precision that set your do the job apart.
No wash/no lyse leukocyte Investigation with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in 1 mM EDTA ( Professional UX/UI enables end users to educate AI styles for expanded applications. (A) The “Train Product” attribute supplies annotation instruments and serious-time model teaching precision.
Gating on these prolonged parameters allows you to rapidly and correctly discover populations of desire and make sure gating system with little or no handbook review. The Attune software program features access to condition-of-the-art device learning Assessment instruments within just an expertly designed user interface, here rendering it extremely simple to operate.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, supplying both spectral unmixing and conventional flow capabilities for thorough and flexible cell Examination.
Agilent Price Promise assures you a minimum of a decade of instrument use through the day you buy. If not, We're going to credit score you Using the residual price of the procedure toward an upgraded model.
What appeared to be a homogeneous populace is actually far more assorted—an Perception that ought to be considered when interpreting effects.